OZ Biosciences
LentiBlast Kit
LB01500
We are interested in enhancing the killing capacity of HIV-specific CD8 T cells by reengineering TCRs expressed by these cells. We use purified CD8 T cells from healthy donors to transduce with the different lentiviral constructs, and use the LentiBlast kit to improve transduction efficiency.
Cell Transduction
Purified Human CD8 T cells
Cells are transduced with the desired construct with 0.5 microliters of solution A and 0.5 microliters of solution B per ml. Cells are plated in a 48 well, and spun down for one hour.
Make sure you titrate the volume needed for each solution.
Great transductions: 30-40% for primary cells, which is difficult to get. Sometimes polybrene can give the same results, but it's more toxic to the cells.
N/A
Great transductions, low toxicity
None
Great kit