Staining Mouse MSC With Anti-CD105 APC

February 23, 2017

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

anti-mouse CD105 APC

Catalog Number:

120414

This antibody was used to phenotype mouse bone marrow derived mesenchymal stem/stromal cells. This antibody was chosen because it is a commonly used clone for CD105, APC is bright and works well with other fluorophores in the staining panel, and Biolegend had the best price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse MSC

Primary Incubation

1:100 dilution in 100uL total staining volume for 15 minutes at room temperature.

Blocking Agent

2% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

N/A

Results Summary

All cells were positive for CD105, as expected. Positive cells were strongly stained compared to unstained cells, and would be fully resolved from negative cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well

The Bad

N/A

The Bottom Line

Will continue to use this antibody.

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