Miltenyi Biotec
Lineage Depletion Kit, mouse
130-090-858
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Our lab has generated a mouse model that expresses three transgenic human host factors and can support HIV infection. We are interested in understanding protective immune responses that could protect against HIV infection in these mice. To understand the role that CD8 T cell responses might have against HIV infection, we reprogram their precursor cells at stem cell level by introducing TCRs that are known to have potent anti-HIV activity. We use this kit to deplete all lineage committed cells from bone marrow.
Cell depletion
Mouse bone marrow
Use the kit instructions: It's a two-step labeling process, where first cells are stained with biotynilated antibodies for lineage markers, then anti-biotin beads are added to the cells to capture them in the separation column
Follow their recommendations; everything has been optimized pretty well
Has worked very well for us
N/A
Good depletion
None
I recommend this kit