Miltenyi Biotecc
CD8 MicroBeads, human
130-045-201
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We are interested in enhancing the killing capacity of HIV-specific CD8 T cells by reengineering TCRs expressed by these cells. We use purified CD8 T cells from donors with known HLA types, to transduce them with the desired lentiviral construct and test their in vitro activity against HIV infected cells.
Purification of CD8 T cells
Whole human PBMC
We follow the directions that come with the kit. It's a standard 15 min incubation at 4 degrees with beads, following the cell to bead ratios recommended. Cell are then washed and ran through a purification column.
Follow the kit instructions
Great separation with high purity (>95%)
N/A
Works very well
None
I recommend this kit.