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PE anti-human CD45RO Antibody
304206
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Browse Similar Products: CD45RO Flow Cytometry Antibodies
Our lab has developed an in vivo humanized mouse model consisting of injecting human PBMC into NSG (immunocompromised) mice to study HIV pathogenesis and test therapeutic agents. Studies investigating different latency reversing agents and treatments involve a detailed analysis of different cell populations in the spleen including memory and effector CD8 and CD4 T cells. Antibodies distinguishing these populations are needed for this purpose.
Flow Cytometry
Human PBMC gated on CD8 T cells
1:100 for 30 min at 4 degrees Celcius
None
Flow cytometry
Antibody works. It is hard to gate on positive and negative populations given that the transition is not very distinct, but with good controls it is possible to separate these populations correctly.
N/A
It work.
Not two clear populations; more like a smear
I will keep using this antibody