Invitrogen by ThermoFisher Scientific
Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 594
A11042
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This antibody has been tested in various mouse fibroblast cell lines and has consistently shown excellent results.
Immunofluorescence
Mouse fibroblasts, various treatment conditions
1:2000 for 1 hour
BSA
1:1000 for 1 hour
None
Confocal microscopy
It had been quite challenging to use my antibodies of chicken origin by western blot so I designed a few pilot experiment to ask my questions by microscopy. Using this antibody in conduction with the primary antibodies gave clean reproducible results. I am pleased with this.
N/A
Provides good images and is easy to optimize
Incubation time is very crucial!
Great antibody!