Invitrogen by ThermoFisher Scientific
Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647
A21245
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This antibody was primarily applied in co-localization studies to stain proteins expressed ectopically in human cell lines.
Immunofluorescence
Transfected and fixed HEK 293
1:1000 for 3 hours
BSA
1:1000 for 1 hour
None
Confocal microscopy
This antibody worked well in my studies and particularly looked good in cells with punctate morphology.
N/A
It was relatively easy to optimize
Not particularly vibrant, but that is understandable due to the wavelength
I would recommend this antibody!