Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 594

February 16, 2017

Medical Microbiology and Immunology
The University of Toledo HSC
Graduate Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen by ThermoFisher Scientific

Product Name:

Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594

Catalog Number:

A11037

This secondary antibody has been applied to fixed cells for immunofluorescence microscopy. The results have been good so far.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Fixed HEK 293 or fibroblast cells

Primary Incubation

1:1000 V5 for 3 hours

Blocking Agent

BSA

Secondary Incubation

1:1000 for 1 hour

Tertiary Incubation

None

Detection

Confocal microscopy

Results Summary

Using this antibody at the indicated dilution has given adequate confocal images. The staining is not as bright as the AF 488 counterpart, but with proper optimization and patient imaging, this antibody can work great!

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well

The Bad

Requires extra care in imaging

The Bottom Line

Great choice of secondary antibody

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