Invitrogen by ThermoFisher Scientific
Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488
A11029
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This secondary antibody was used for immunofluorescence microscopy in both mouse and human cells. Overall, it works quite well and gives a clean staining.
Immunofluorescence
Fixed mouse and human cells
1:2000 for 3 hours
BSA
1:1000 for 1 hour
N/A
Confocal microscopy
With each use, I am able to achieve consistently vibrant staining with this antibody.
Easy to optimize, images well.
Incubation must be strictly timed!
Highly recommend!