The Good
Simple protocol, inexpensive, easy to use, reliable. Dye is separated into multiple vials, providing additional longevity from a single kit.
The Bad
Like all flow cytometry protocols, make sure to optimize the dilution prior to use on prescious samples.
The Bottom Line
I recommend this product to anyone who needs a reliable live/dead stain for flow cytometry protocols that involve fixing. We use this reagent in panels comprised of 7+ colors and have never experienced any problems with the product. Multiple other color options (other than blue) are available from the company to best fit the needs of different multi-color experiments.