Biolegend
FITC anti-mouse IFN-γ Antibody
505806
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Splenocytes extracted from brain tumor-bearing mice, gated for live cells, CD45+, CD3+, CD8+ and IFNg. The experiment was perform to evaluate the release of IFNg after treatment.The Ab was choose for the the good quality/price ratio
Flow Cytometry
Mouse Splenocytes
30min
Fc Ab
N/A
Flow - Fortessa
Cells are been stimulate in vitro for 4h before the staining.after cell extraction, blocking with Fc Block, and extracellular staining proceed with the intracellular protocol.Use INFg 1:200.
Good color
High concentration to use (1:200)
Fine Ab for IFNg staining