Biolegend
PE anti-mouse CD107a (LAMP-1) Antibody
121611
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Splenocytes extracted from brain tumor-bearing mice, gated for live cells, CD45+, CD3+ and CD107a.The experiment was perform to evaluate the changes in LAMP3 population after treatment.The Ab was choose for the the good quality/price ratio.
Flow Cytometry
Mouse Splenocytes
30min
Fc Ab
N/A
Flow - Fortessa
After cell extraction and blocking with Fc Block, wash your sample 2x with washing buffer, stain for 30min RT with CD107a 1:200, wash 2x, fix in PFA2% or proceed with intracellular staining protocol.Spleenocyte extracted from brain tumor-bearing mice, gated for live cells, CD45low, CD3- and F4/80.poor background, clean populationNice antibody to detect a clear macrophages/microglia population.
Nice population
High concentration to use (1:200)
Good antibody for CD107a, tested on splenocytes