Great anti-PD1 Antibody

Medical Oncology
DFCI
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-mouse CD279 PerCP-eFluor710

Catalog Number:

46-9981-82

Splenocytes extracted from brain tumor-bearing mice, gated for live cells, CD45+, CD3+, CD8+ and PD1+ low and high. The experiment was perform to evaluate the changes in PD1 population after treatment. The Ab was choose for the the good quality and color.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Splenocytes

Primary Incubation

30min

Blocking Agent

Fc Ab

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow - Fortessa

Results Summary

After cell extraction and blocking with Fc Block, wash your sample 2x with washing buffer, stain for 30min RT with aPD1 1:100, wash 2x, fix in PFA2% or proceed with intracellular staining protocol. Splenocytes extracted from brain tumor-bearing mice, gated for live cells, CD45+, CD3+, CD8+, PD1+

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Perfect staining

The Bad

None

The Bottom Line

Really good Ab to detect PD1 in mouse

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