Good Mouse CD3 Antibody

February 13, 2017

Medical Oncology
DFCI
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 785 anti-mouse CD3 Antibody

Catalog Number:

100232

CD3+ cells of a tumor-bearing mouse. The experiment was perform to evaluate the changes in CD3 population after treatment.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Splenocytes

Primary Incubation

30min

Blocking Agent

Fc Ab

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow - Fortessa

Results Summary

After cell extraction and blocking with Fc Block, wash your sample 2x with washing buffer, stain for 30min RT with CD3 1:200, wash 2x, fix in PFA2% or proceed with intracellular staining protocol

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Nice color

The Bad

Need high concentration

The Bottom Line

Reasonable good Ab

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