mCD45 BV605 Antibody

Medical Oncology
DFCI
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 605™ anti-mouse CD45 Antibody

Catalog Number:

103140

Ab used to detect CD45+ cells in isolated PBMCs from mice after brain tumor injection.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Splenocytes

Primary Incubation

30min

Blocking Agent

Fc Ab

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow - Fortessa

Results Summary

After cell extraction and blocking with Fc Block, wash your sample 2x with washing buffer, stain for 30min RT with CD45 1:100, wash 2x, fix in PFA2% or proceed with intracellular staining protocol

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background

The Bad

None

The Bottom Line

Great Ab to detect CD45 cells.

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