Two Independent, Simultaneous PCR Reactions

Boston University
Center for Regenerative Medicine
Postdoctoral Associate

Overall

Performance

Ease-of-Optimization

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Company:

Eppendorf

Product Name:

Mastercycler Nexus X2

Catalog Number:

6337000027

This PCR machines allows researchers to run two independent PCR reactions. Therefore, the aim of this experiment was to use the mastercycler to run two reactions with different PCR cycles. The goal was to use the PCR products and run them on the gel. This equipment was recommended by colleagues. Easy to operate and set the cycles.

Experimental Design and Results Summary

Application

PCR reaction

Starting Material

DNA extracted from cell pellet

Protocol Overview

The PCR settings were set as per the genes of interest to be amplified.

Tips

Not applicable as the machine is very easy to operate.

Results Summary

PCR products showed successful amplification of the DNA template when run on the gel.

Features Summary

Two independent reactions can be run at the same time.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to operate and set the cycles on both heat blocks.

The Bad

None

The Bottom Line

Very intuitive PCR machine, great for busy labs.

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