AEC Substrate Use For Immunohistochemistry

Northwestern University
Immunology
Grad Student

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Quality of Results

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Company:

Vector

Product Name:

AEC Peroxidase (HRP) Substrate Kit, 3-amino-9-ethylcarbazole

Catalog Number:

SK-4200

The goal of the project was to stain spleen sections for different immune cells during an immune response. The product was used as a substrate for immunohistochemistry staining of spleen sections.

Experimental Design and Results Summary

Application

Immunohistochemistry

Starting Material

Mouse spleen tissue

Protocol Overview

3 dropper bottles: Buffer, AEC, Hydrogen peroxide solution. In 5 ml of water, the dropper solutions are mixed and added to slides

Tips

None

Results Summary

Distinct and dark staining

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use, clearly marks positive cells

The Bad

None

The Bottom Line

Good substrate for immunohistological staining of tissue sections

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