Mouse TNF alpha ELISA

Harvard
Harvard Medical School
Postdoctoral Researcher

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Company:

eBioscience

Product Name:

Mouse TNF alpha ELISA Ready-SET-Go

Catalog Number:

88-7324-86

I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.

Experimental Design and Results Summary

Application

TNF-alpha detection in biological samples

Starting Material

Mouse tissue homogenates

Protocol Overview

Overnight incubation at 4 degree with capture antibodyBlocking with 200 µL/well of 1X ELISA/ELISPOT DiluentOvernight incubation assay supernatant at different dilutions1 hour incubation with detection antibody at room temperatureELISA-liquid TMB substrate development

Tips

Standardize sample dilution first as per the kit detection range

Results Summary

High TNF-alpha levels were detected in mice tissue homogenates.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Nice TNF-alpha detection ELISA kit

The Bad

None

The Bottom Line

Nice TNF-alpha detection ELISA kit, gives reliable results when used within expiry date of the kit.

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