Mouse IL-4 ELISA

Harvard
Harvard Medical School
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Mouse IL-4 ELISA Ready-SET-Go

Catalog Number:

88-7044-86

I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.

Experimental Design and Results Summary

Application

IL-4 detection in biological fluids

Starting Material

Mouse sera

Protocol Overview

Overnight incubation at 4 degree with capture antibodyBlocking with 200 µL/well of 1X ELISA/ELISPOT DiluentOvernight incubation assay supernatant at different dilutions1 hour incubation with detection antibody at room temperatureELISA-liquid TMB substrate

Tips

Standardize sample dilution first as per the kit detection range

Results Summary

IL-4 levels were detected in mice sera and found to be higher in KO mice that appeared to be decreasing after drug administration.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Nice and efficient IL-4 detection

The Bad

None

The Bottom Line

NIce and efficient IL-4 detection from mice sera samples. Make sure to use the kit within its expiry date.

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