Cell Signaling Technology
NF-κB p65 (D14E12) XP® Rabbit mAb
8242S
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I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's system using anti-mycobacterial ESAT6 binding peptides.
Western Blot
Mouse whole cell lysates
1:1000 at 4 degrees for overnight incubation
5% skimmed milk in 1X TBST
1:3000 at room temperature for 2 hour in 5% skimmed milk
NA
High levels of total NF-kB p65 protein were detected in cell lysates.
Good signal with primary antibody
None
Good antibody for total p65 protein detection. Important to follow Cell Signaling Technology instruction sheet.