Excellent B-Actin Antibody

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Cell Signaling Technology

Product Name:

β-Actin (13E5) Rabbit mAb (HRP Conjugate)

Catalog Number:

5125S

I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's system using anti-mycobacterial ESAT6 binding peptides.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse whole cell lysates

Primary Incubation

1:1000 at room temperature for 30 minutes

Blocking Agent

5% skimmed milk in 1X TBST

Secondary Incubation

None

Tertiary Incubation

NA

Detection

Western Blot

Results Summary

High levels of b-actin were detected by using this antibody. Highly specific and efficient.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Good signal and no need for secondary antibody

The Bad

None

The Bottom Line

This antibody is ideal for detecting fast and efficient detection of b-actin protein in Western Blots.

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