Great Source For Bone Marrow Aspirate

Luminex
Amnis - Biology

Overall

Quality of Results

Ease-of-Optimization

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Company:

AllCells

Product Name:

Whole Bone Marrow Aspirate

Catalog Number:

ABM001-1

The project I am currently working on uses immunophenotyping to identify different subsets of cells in the Bone Marrow. In order to do this we need fresh Bone Marrow Aspirate samples. Bone Marrow Aspirate is not easy to come by like Peripheral Blood and the quality of the pull can also be an issue as the Bone Marrow can be diluted by blood. Over the last year we have received over a dozen Bone Marrow Aspirates for AllCells and all have been of good quality. My lab has also received Bone Marrow Aspirates from another company (I will not mention the name of this other company in this review), the quality of the Bone Marrow Aspirate was probably about the same; however, the price was more, their customer service was lacking and the container that we received the sample in was hard to work with. AllCells on the other hand was less expensive, has great customer service and sample come in a vial/tube that is easy to work with.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

Human Bone Marrow

Protocol Overview

The Bone Marrow Aspirate was labeled with CD markers. The red cells were lysed and the labeled white blood cells were run/analyzed on a Flow Cytometer.

Tips

Use the Bone Marrow Aspirate the day you receive it as it is a fresh sample.

Results Summary

We were able to identify all the populations we were looking for in the Bone Marrow Aspirate.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

This is a good source for fresh Bone Marrow Aspirate.

The Bad

You can't always get a sample on the day you wanted it and you usually need to give them a week and a half notice (since they need to schedule a donor and a physician to perform the procedure).

The Bottom Line

If you need fresh Bone Marrow Aspirate AllCells is a good source. They are easy to work with and the Bone Marrow Aspirate is of good quality.

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