Good Antibody To Identify CCR7+ Cells

Pulmonary
Harvard Medical School
Post Doc

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Company:

BD Biosciences

Product Name:

Alexa Fluor® 647 Rat Anti-Human CD197 (CCR7)

Catalog Number:

557734

This antibody was used to phenotypically characterize PBMCs of children with arthritis, Since we only had a limited amount of sample we had to chose reliable antibodies that worked very well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human peripheral blood

Primary Incubation

50ul of blood were incubated with a 1:3 dilution of the antibody for 30 minutes. Then cells were lysed with BD lysing solution and washed twice with PBS. Cells were then resuspended in 300l of FACS buffer and taken to flow cytometry

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

This is a good antibody to identify CCR7+ cells in human peripheral blood.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good staining of CCR7+ cells

The Bad

Antibody can only be used in 1:3 dilution. If further diluted the staining is not as clear

The Bottom Line

This is a good antibody to identify CCR7+ cells.

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