BD Biosciences
PE Mouse Anti-Human CD25 Clone M-A251
555432
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The goal of this experiment was to identify CD25+ T cells in peripheral blood of children with juvenile idiopathic arthritis. Since we only had a limited amount of sample we needed antibodies that worked very well and reliably.
Flow Cytometry
Human peripheral blood
50ul of peripheral blood were incubated with the antibody for 30 minutes. Then red cells were lysed using BD lysing solution. After 2 wash steps with 1 ml of PBS, stained cells were resuspended in 300ul FACS buffer and taken directly to flow cytometry
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Flow cytometry
This is a good antibody to detect CD25+ T cells in peripheral blood.
N/A
Good stating of CD25 on PBMCs
Nothing
This is a reliable antibody to identify CD25+ human T cells