Time Course Effect Of Chemoattractant On Alpha Actin Expression

January 18, 2017

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Sigma Aldrich

Product Name:

Monoclonal Anti-alpha Smooth Muscle Actin Clone 1A4

Catalog Number:

A2547

This antibody is specific for single isoform alpha actin. It is one of the important cytoskeletol protein responsible for cell motility. Here we checked the time course effect of chemoattractant on smooth muscle cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse cell lysate

Primary Incubation

1:500 in 5% BSA prepared in 1x TBST

Blocking Agent

5% milk prepared in 1x TBST

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL solution

Results Summary

We found time dependent increase in alpha actin expression.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background and the signal is prominent.

The Bad

None

The Bottom Line

Highly recommended antibody for western blot.

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