Santa Cruz Biotechnology
Galpha12
sc-409
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Gα12 a α subunits belongs to a class of heterotrimeric G proteins along with GαS, Gαi/o, and Gαq. Like other G proteins it involves in the binding of GTP which is latter hydrolyzed to GDP protein to the inactive state. Here we checked the time course effect of chemoattractant on marophages.
Western Blot
Mouse cell lysate
1:500 in 5% BSA prepared in 1x TBST
5% milk prepared in 1x TBST
1:4000 prepared in 1x TBST
None
ECL solution
We found time dependent increase of Galpha12 expression by chemoattractant.
N/A
Very good for western blot
Highly recommended for western blot.