Santa Cruz Biotechnology
Galpha13
sc-410
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G-protein-coupled receptor is a 7-transmembrane domain receptor. Galpha13 is known for activating a family of Rho guanine-nucleotide exchange factors called RhoGEFS which subsequently leads to activation of the small GTPase RhoA in response to ligand binding by several G protein-coupled receptors (GPCRs).Here we checked its role in macrophages by chemoattractant.
Western Blot
Mouse cell lysate
1:1000 in 5% BSA prepared in 1x TBST
5% milk prepared in 1x TBST
1:4000 prepared in 1x TBST
None
ECL solution
We found that time dependent increase of Galpha13 expression by chemoattractant.
N/A
No background and the signal is prominent.
Highly recommended antibodies to study the G protein functions by western blot.