Time Course Effect Of Chemoattractant On Galpha11 Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology

Product Name:

Galpha11

Catalog Number:

sc-394

G alpha 11 is a heterotrimeric protein which carries information from cell surface receptors to intracellular effectors. Here we checked its role in macrophages by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse cell lysate

Primary Incubation

1:500 in 5% BSA prepared in 1x TBST

Blocking Agent

5% milk prepared in 1x TBST

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL solution

Results Summary

We found time dependent increased expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

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Image Gallery

Summary

The Good

No background and the signal is prominent.

The Bad

None

The Bottom Line

Highly recommended antibody to study the G proteins functions.

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