BD Biosciences
BUV737 Mouse Anti-Human CD14
564445
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The general aim of my research is to study the antigen-presenting potential of human monocyte-derived dendritic cells (moDCs). The goal of this particular experiment was to detect and enrich CD14+ cells from human peripheral blood. This specific product was used because of the fluorochome-conjugate, BUV737, which does not bleed into other channels (except slightly into PerCP/Cy5.5 and APC/Cy7).
Flow Cytometry
Human peripheral blood
1:250, 20 mins, 4 C in 2% FBS in PBS with 2mM EDTA
Human TruStain FcX (Biolegend)
N/A
355nm laser on BD LSRII
CD14+ cells in human peripheral blood were found to be at a frequency ranging between 20-40% depending on the donor. Magnetic bead enrichment of the CD14+ cells lead to ~85-90% enrichment.
Little to no compensation needed with other channels
None
Excellent product for use on the UV laser!