Cell Signaling Technology
phospho-Akt Antibody
4060S
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I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's system using anti-mycobacterial ESAT6 binding peptides.
Western Blot
Mouse whole cell lysates
1:1000 at 4 degrees for overnight incubation
5% skimmed milk in 1X TBST
1:3000 at room temperature for 2 hour in 5% skimmed milk
NA
phospho-Akt protein was detected with stimulation of macrophages with antigen.
Specific antibody without background signal
None
Gives nice signal with Bio-Rad anti-Rabbit-HRP secondary antibody.