Great CD21/CD35 (CR2/CR1) Antibody

January 04, 2017

Microbiology
Medical College of Wisconsin
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Pacific Blue™ anti-mouse CD21/CD35 (CR2/CR1) Antibody

Catalog Number:

123414

We study virus-host interactions using a mouse model of infection. Here we were interested in what happens to B cell populations, specifically marginal zone B cells, following infection. Biolegend is our go-to company for flow cytometry reagents, and this CD21 antibody works great to distinguish follicular from marginal zone B cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:400 for 25 min at 4C

Blocking Agent

Fc blocking antibody

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII

Results Summary

Great antibody for phenotyping B cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Reliable, easy to use

The Bad

n/a

The Bottom Line

Great antibody

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