Anti-Mouse CD45 BV711

Medical Oncology
DFCI
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 711™ anti-mouse CD45 Antibody

Catalog Number:

103147

CD45-BV711 was used to gate CD45+ cells extracted from spleenocytes, cLN and TILs of tumor-bearing mice, with the final aim to study the immune system response after treatment. This product has been preferred to other thanks to good price/quality ratio. After cell extraction and blocking with Fc Block, wash your sample 2x with washing buffer, stain for 30min RT with CD45 1:100, wash 2x, fix in PFA2% or proceed with intracellular staining protocol

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Splenocytes

Primary Incubation

30min

Blocking Agent

Fc

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FC Fortessa

Results Summary

Splenocyte extracted from brain tumor-bearing mice, gated for live cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Nice clear population

The Bad

None

The Bottom Line

Really good antibody for CD45+ cells detection.

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