Biolegend
Brilliant Violet 785™ anti-mouse/human CD11b Antibody
101243
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Browse Similar Products: CD11b Flow Cytometry Antibodies
CD11b-BV786 was used to gate CD11b+ of CD45+CD3- cells extracted from spleenocytes, cLN and TILs of tumor-bearing mice, with the final aim to study the macrophages/microglia change after treatment. This product has been preferred to other thanks to good price/quality ratio. After cell extraction and blocking with Fc Block, wash your sample 2x with washing buffer, stain for 30min RT with CD11b 1:100, wash 2x, fix in PFA2% or proceed with intracellular staining protocol
Flow Cytometry
Mouse Splenocytes
30min
Fc
N/A
FC
Splenocytes extracted from brain tumor-bearing mice, gated for live cells, CD45low, CD3- and CD11b+.
Easy to use
Not a really clear population
Good antibody, tested on splenocytes, LN and TILs.