Anti-Mouse CD3 PE-Cy7

Medical Oncology
DFCI
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE/Cy7 anti-mouse CD3 Antibody

Catalog Number:

100220

CD3-PE-Cy7 was used to gate CD3- of CD45+cells extracted from spleenocytes, cLN and TILs of tumor-bearing mice, with the final aim to exclude the T-cell population from the analysis of the innate immune response. This product has been preferred to other thanks to good price/quality ratio. Good antibody anti mouse, tested in spleen, TILs and cLN.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

30min

Blocking Agent

Fc

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Splenocytes extracted from brain tumor-bearing mice, gated for live cells and CD45+.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clear positive population

The Bad

Uses 2:100 dilution

The Bottom Line

Really good.

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