Excellent For Immunostaining

January 03, 2017

pediatrics
University of Mississippi Medical Center
Assistant Professor

Overall

Quality of Results

Ease-of-Optimization

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Company:

Swant Inc

Product Name:

anti-Calbindin, Rabbit polyclonal antibody

Catalog Number:

CB38

Used to label Calbindin+ interneurons in rat free-floating brain sections. Excellent results.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Rat brain tissue section (postnatal day 21), free-floating

Primary Incubation

Diluted 1:400 with 10% normal goat serum in PBS, incubated overnight at 4oC

Blocking Agent

10% of Goat serum with 0.2% triton in PBS

Secondary Incubation

Biotin-labeled anti-Rabbit IgG

Tertiary Incubation

Avidin-Alex488

Detection

Immunofluorescence

Results Summary

Excellent staining. Bright fluorescence immunostaining with very low background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright staining with low background

The Bad

None

The Bottom Line

Works very well for free-floating brain sections. May also work for paraffin-embedded tissue.

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