Alexa 488 E-Cadherin

Cell and Molecular Biology
Northwestern University
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Alexa 488 E-Cadherin Antibody

Catalog Number:

560061

The overall goal of my research is to gain insights into HIV transmission. The specific goal in which the E-Cadherin antibody was used to test the integrity of cellular junctions. This antibody was chosen as it was published to work in variety of Rhesus Macaque tissues.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Rhesus macaque tissue

Primary Incubation

One hour at room temperature

Blocking Agent

PIPES/Formaldehyde fix, Normal Donkey Serum for Blocking

Secondary Incubation

None

Tertiary Incubation

None

Detection

Microscopy

Results Summary

This antibody works great in variety of tissues. Example of rectal tissue is included. The staining is very robust with no bleed through in other channels.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy one our incubation, very bright

The Bad

None

The Bottom Line

Highly recommended for use in Rhesus Macaque female reproductive tract tissues and anal/rectal tissues.

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