Cdc42 Activation Assay

Albert Einstein College of Medicine
Physiology and Biophysics
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Bio Labs

Product Name:

Cdc42 Activation Assay

Catalog Number:

STA-402

The aim of this experiment was to investigate the levels of activated Cdc42 in cytokine stimulated cells. The goal was to pull down activated Cdc42 and detect Cdc42 via Western blot. This product has been used in previous publications, and that is why it was originally selected. After multiple attempts to optimize this assay, we gave up on this kit

Experimental Design and Results Summary

Application

Cdc42 activation assay

Starting Material

Human U2OS osteosarcoma cells

Protocol Overview

This kit is used to pull down activated Cdc42, however the antibody provided did not pick up any endogenous Cdc42 during the primary optimization steps recommended.

Tips

Don't purchase this kit

Results Summary

No results were obtained using this kit.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

None

The Bad

The antibody provided

The Bottom Line

Try a different kit

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