Rabbit VEGF-R2 antibody for Western Blot

December 19, 2016

BlueskyReddit
Physiology and Biophysics
Albert Einstein College of Medicine
Graduate student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Invitrogen by ThermoFisher

Product Name:

VEGF Receptor 2/Flk-1 Antibody (B.309.4)

Catalog Number:

MA5-15157

This antibody works great to detect VEGF-R2 in HUVECs by Western blot analysis. It does not work well for immunofluorescence microscopy

Experimental Design and Results Summary

Applications

Western Blot

Sample

HUVEC lysate

Primary Incubation

Overnight, 1:2000

Blocking Agent

5% milk

Secondary Incubation

1 hour, 1:10000

Tertiary Incubation

NA

Detection

ChemiDoc™ Touch Imaging System

Results Summary

Western blot analysis to detect endogenous levels of VEGFR2 in HUVEC samples.

DOI or PMID #

NA

Additional Notes

Does not work well for microscopy

Related Categories

Image Gallery

Summary

The Good

Clean bands

The Bad

Overnight incubation

The Bottom Line

Reliable antibody for Western blot detection of VEGFR2

Share your experience with other scientists. Write a Review! »

Join the discussion