GRP94 antibody

Environ. Med.
University of Rochester
Postdoc

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Company:

Santa Cruz Biotechnology

Product Name:

GRP 94 Antibody (H-10)

Catalog Number:

sc-393402

We performed immunoblotting analysis using lysates from exosomes isolated from human plasma. Exosomal marker negative control GRP 94 was analysed by immunoblot analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human exosome lysates

Primary Incubation

1:1000 dilution in 5% milk

Blocking Agent

5% milk at room temperature for 1 hr

Secondary Incubation

1:10,000 dilution in 5% milk

Tertiary Incubation

N/A

Detection

ECL based detection using Bio-Rad Chemidoc MP

Results Summary

We did not detect any GRP94 in our exosomal fraction. Which is a negative control for exosomal proteins. This ab works in human samples.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works in human samples

The Bad

Noted none

The Bottom Line

Works for WB analysis of GRP94 in human samples.

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