Calnexin Antibody

Environ. Med.
University of Rochester
Postdoc

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Quality of Results

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Company:

Santa Cruz Biotechnology

Product Name:

Calnexin Antibody (H-70)

Catalog Number:

sc-11397

We performed immunoblotting analysis using lysates from exosomes isolated from human plasma. Exosomal negative control marker Calnexin was analysed by immunoblot analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human exosome lysates

Primary Incubation

1:1000 dilution in 5% milk

Blocking Agent

5% milk at room temperature for 1 hr

Secondary Incubation

1:10,000 dilution in 5% milk

Tertiary Incubation

N/A

Detection

ECL based detection using Bio-Rad Chemidoc MP

Results Summary

We were unable to detect calnexin in our samples which is a negative control for exosomal proteins. Antibody work good for human samples.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works in human samples

The Bad

Noted none

The Bottom Line

Works in human samples for WB analysis

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