Rab-5B Antibody

December 22, 2016

Environ. Med.
University of Rochester
Postdoc

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Company:

Santa Cruz Biotechnology

Product Name:

Rab 5B Antibody (F-9)

Catalog Number:

sc-373725

Info: View Product View Product Specs

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We performed immunoblotting analysis using lysates from exosomes isolated from human plasma. Exosomal marker Rab-5B was analysed by immunoblot analysis.

Experimental Design and Results Summary

Applications

Slot-blot analysis

Sample

Human exosome lysates

Primary Incubation

1:1000 dilution in 5% milk

Blocking Agent

5% milk at room temperature for 1 hr

Secondary Incubation

1:10,000 dilution in 5% milk

Tertiary Incubation

N/A

Detection

ECL based detection using Bio-Rad Chemidoc MP

Results Summary

Works well for detection of Rab-5B in human exosomal fraction.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works in human exosomal lysates

The Bad

Noted none

The Bottom Line

Works in human exosomal fraction

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