Santa Cruz Biotechnology
Alix Antibody (1A12)
sc-53540
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We performed immunoblotting analysis using lysates from exosomes isolated from human plasma. Exosomal marker Alix was analysed by immunoblot analysis.
Slot-blot analysis
Human exosome lysates
1:1000 dilution in 5% milk
5% milk at room temperature for 1 hr
1:10,000 dilution in 5% milk
N/A
ECL based detection using Bio-Rad Chemidoc MP
Exosomal marker Alix was detected by slot-blot analysis in exosomal fraction.
Works in human exosomal lysates
Noted none
Works for immunoblot analysis using human exosomal lysates