TSG 101 Antibody

December 19, 2016

Environ. Med.
University of Rochester
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

tsg 101 Antibody (C-2)

Catalog Number:

sc-7964

Info: View Product View Product Specs

Browse Similar Products: Tsg 101 Antibodies

We performed immunoblotting analysis using lysates from exosomes isolated from human plasma. Exosomal marker Tsg 101 was analysed by immunoblot analysis.

Experimental Design and Results Summary

Applications

Slot-blot analysis

Sample

Human exosome lysates

Primary Incubation

1:1000 dilution in 5% milk

Blocking Agent

5% milk at room temperature for 1 hr

Secondary Incubation

1:10,000 dilution in 5% milk

Tertiary Incubation

N/A

Detection

ECL based detection using Bio-Rad Chemidoc MP

Results Summary

We were able to detect TSG 101 in our exosomal lysates.

DOI or PMID #

N/A

Additional Notes

https://www.scbt.com/scbt/product/tsg-101-antibody-c-2

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Summary

The Good

Works in human exosomal lysates

The Bad

Noted none

The Bottom Line

Works for immunobolot analysis of TSG 101

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