Exosome marker CD81

December 16, 2016

Environ. Med.
University of Rochester
Postdoc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

SystemBio

Product Name:

Anti-CD81 Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody

Catalog Number:

EXOAB-CD81A-1

 

Browse Similar Products: CD81 Western Blot Antibodies

We performed immunoblotting analysis using lysates from exosomes isolated from human plasma. CD81 a marker present in exosomal fractions were detected in our samples as well.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human exosome lysates

Primary Incubation

1:1000 dilution in 5% milk

Blocking Agent

5% milk at room temperature for 1 hr

Secondary Incubation

1:10,000 dilution in 5% milk

Tertiary Incubation

N/A

Detection

ECL based detection using Bio-Rad Chemidoc MP

Results Summary

CD81 levels were detected in exosomal lysates.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works in human exosomal lysates

The Bad

Noted none

The Bottom Line

Works in human samples for immunoblot analysis

Share your experience with other scientists. Write a Review! »

Join the discussion