Biolegend
PE/Cy7 anti-mouse TNF-α Antibody
506324
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Browse Similar Products: TNF-alpha Flow Cytometry Antibodies
I am interested in studying the immune system of the murine colonic lamina propria. Therefore, as a first step we had used a panel of antibodies to check the frequency of different types of immune cells.
Flow Cytometry
Mouse colon tissue sample
1:200 dilution; 20 min at 4 deg C
FcBlock for 5 min
None
TNF alpha positive cells are clearly distinguished with this antibody
N/A
For TNF alpha staining, intracellular antigen staining protocol (eBioscience) was followed
Very clear staining with no background problem
Highly recommened!