Good Antibody To Detect IL33 By Immunostaining

Animal Biochemistry
National Dairy Research Institute
Scientist

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Company:

R&D systems

Product Name:

IL-33 antibody

Catalog Number:

AF3625

In the present study, IL33 proteins were immunostained in the pancreatic sections of cholesterol induced diabetic and non-diabetic rats. Further, primary antibodies were identified by alexa fluor labeled anti species secondary antibody.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Rat pancreatic tissue

Primary Incubation

1:100 overnight at 4 degree Celsius

Blocking Agent

3% BSA solution containing 0.5 % normal goat serum for 1 hr

Secondary Incubation

1:500 for 1 hour

Tertiary Incubation

None

Detection

Fluoresecnce microscopy

Results Summary

There was bright staining of IL-33 proteins as detected by strong specific signal of stained IL33 proteins under fluorescent microscope.

DOI or PMID #

N/A

Additional Notes

No

Related Categories

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Summary

The Good

Antibody specifically stained IL-33 only

The Bad

None

The Bottom Line

Very good Antibody for immunostaining of IL-33

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