Good Antibody For CD25 Staining

January 06, 2017

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

eBioscience

Product Name:

Anti-Human CD25 PE

Catalog Number:

12-0259-80

I used this antibody to characterize human Treg based on CD4+CD25+CD127low staining.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:400 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

CD25 staining was somewhat weak. However, it is consistent and reproducible.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Ease of optimization and reproducible results

The Bad

Somewhat weak staining

The Bottom Line

Good choice

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