Good antibody for mouse CD11c staining

January 05, 2017

BlueskyReddit
Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse CD11c eFluor® 450

Catalog Number:

48-0114-80

I used this antibody to characterize CD11c+ DCs in mouse spleen and thymus.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Thymocytes

Primary Incubation

1:200 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

I got somewhat weak staining of CD11c, but it was reproducible.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Cheap and reproducible results

The Bad

Somewhat weak staining

The Bottom Line

Good choice

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