Excellent Flow Cytometry Antibody For Mouse IFN-Gamma

December 28, 2016

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

eBioscience

Product Name:

Anti-Mouse IFN gamma PE

Catalog Number:

12-7311-41

I used to this antibody to stain IFN-g+ T-cells in mouse splenocytes after stimulation with PMS-Ionomycin cocktail.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:400 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

I always get a nice IFN-g staining and distinct IFN-g+ population of T-cells which is of presentable quality.

DOI or PMID #

NA

Additional Notes

I used Foxp3 transcription Fix/Permeabilation Kit from Tonbo Bioscience for fixation and permeabilization and PMA cocktail containing protein transport inhibitors

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Nothing

The Bottom Line

Best choice

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