Excellent Flow Cytometry Antibody For Mouse Helios

December 15, 2016

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

eBioscience

Product Name:

Anti-Helios PE

Catalog Number:

12-9883-41

I used to this antibody to stain Helios+ Treg-cells in mouse spleen, thymus and lymph nodes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse T-cells

Primary Incubation

1:400 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

I always get a nice staining of Helios+ population of Treg-cells which is of presentable quality.

DOI or PMID #

NA

Additional Notes

I used Foxp3 transcription Fix/Permeabilation Kit from Tonbo Bioscience for fixation and permeabilization

Related Categories

Image Gallery

Summary

The Good

Ease of optimization and reproducible results

The Bad

Expensive

The Bottom Line

Worth buying it

Share your experience with other scientists. Write a Review! »

Join the discussion